DISEÑO IN SILICO DE PÉPTIDOS ANTIMICROBIANOS DERIVADOS DE FAGOS PARA LA ERRADICACIÓN DE ENTEROCOCCUS FAECIUM
DOI:
https://doi.org/10.47187/cssn.Vol15.Iss1.276Palabras clave:
Fagoterapia, péptidos antimicrobianos (PAMs), Enterococcus faecium, erradicación de biofilms, resistencia a los antibióticosResumen
Introducción: Enterococcus faecium ha generado creciente preocupación debido a su asociación con infecciones nosocomiales y resistencia a antibióticos, siendo responsable de la mayoría de las infecciones por enterococos resistentes a la vancomicina (VRE) y formando biofilms que confieren resistencia a antibióticos como linezolid y tigeciclina. Materiales y Métodos: Se diseñaron péptidos derivados de fagos vB_Efm_LG62 y vB_EfKS5 para E. faecium. Se utilizó el servidor AMP Scanner vr.2 para identificar candidatos a péptidos antimicrobianos (PAMs), y luego los servidores CellPPD, dPABB y ToxinPred para evaluar la penetración en células bacterianas, la erradicación de biofilms y la toxicidad, respectivamente. Resultados: Se identificaron proteínas con actividad antibacteriana que sirvieron como base para la identificación de PAMs, los cuales mostraron buena penetración celular, capacidad para erradicar biofilms y baja toxicidad. Discusión: Los PAMs derivados de los fagos presentan dominios HNH y metaloproteasas dependientes de ATP, además de actividades putativas de factor sigma y NAMLAA asociadas con la lisis bacteriana o la replicación de fagos. Conclusión: El diseño in silico de péptidos de fagos ofrece una solución prometedora para tratar infecciones por E. faecium y otras bacterias, resaltando el potencial de los PAMs para avanzar en la terapia antimicrobiana ante la resistencia a los antibióticos.
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